Shown are representative western immunoblot analyses of changes in total protein levels of cytochrome C a and Sod 2 b ; and changes in phospho- and total protein levels of STAT3 c and H2AX d. Female mice 5—6 weeks old were irradiated as previously described. Please enable JavaScript to access the full features of the site or access our non-JavaScript page. Several of these pathways, that is, DNA damage repair, cell cycle, apoptosis, oxidative stress response, are dysregulated in Fus1 KO intestinal epithelium after irradiation. Shown are graphs of the mean BrdU incorporation normalized to sham-irradiated control with S. 
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Remember your video choices. Previous Article Next Article. Watch artist interviews here. Shown are graphs of the mean BrdU incorporation normalized to sham-irradiated control with S.
Cellular oxidative stress response mediates radiosensitivity in Fus1-deficient mice
Party e Kouchik 2. Back to tab navigation Fetching data from CrossRef. Reproduced material should be attributed as follows: Back to tab navigation. Pyridoxamine treatment improves antioxidant response in Fus1 KO cells Formation of ROS occurs immediately after irradiation, continues for several hours, and results in oxidative Koucik damage. Pyridoxamine protects intestinal epithelium from ionizing radiation-induced apoptosis.
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In this study, Fus1 KO cells have lower basal levels of antioxidant proteins cytochrome C and Sod 2 compared with WT cells Figures 6a and bleft kouchii.
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Fetching data from CrossRef. Log in with Facebook. Biological consequences of oxidative stress-induced DNA damage in Saccharomyces cerevisiae. All animal experiments were performed according to a protocol approved by the Institutional Animal Care and Use Committee.
To further confirm the role of oxidative reactions in mechanisms of radiosensitivity of Fus1 KO epithelial cells, we used another inhibitor of oxidative reactions, Sod 2 mimetic Tempol. This may kouchii some time to load. Interestingly, we also observed a mitigation effect of PM treatment, that is, when PM was added to the cells shortly after irradiation.
Party e Kouchik 2 0: To determine the mechanisms whereby Fus1 modulates cellular radiosensitivity, we utilized Fus1 KO mouse model and inhibitors of oxidative pathways with different mechanisms of actions, pyridoxamine PM and Tempol. Please select a valid image file. Always play videos fullscreen. Quantitative analyses of band d shown below the corresponding blots were performed using ImagJ software; the values were normalized to loading control visualized by stain-free gels Bio-Rad, Inc.
Footnotes Edited by G Dewson.
Treated cells were incubated for 0, 0. This work is licensed under a Creative Commons Attribution 4. XX is the XXth reference in the list of references. Stat3 protects against Fas-induced liver injury by redox-dependent and -independent mechanisms.
Western immunoblot analysis Treated cells were incubated for 0, 0.
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